Mass Spectrometry and Chromatography

Super Critical Fluid Chromatography

Supercritical fluid chromatography (SFC)  is the type of normal phase chromatography  that  require superficial fluid as mobile phase  like carbon dioxide. It is used for the evaluation and purification of thermally labile molecules  low to moderate molecular weight and for the separation of chiral compounds. The  of principles of super critical fluid chromatography are similar to high performance liquid chromatography, it uses carbon dioxide as the mobile phase, therefore the pressure should be maintained throughout the entire chromatographic flow path. Sometimes super fluid chromatography is called convergence chromatography because supercritical phase represent a state where gas and liquid properties converge.