Super Critical Fluid Chromatography
Supercritical fluid chromatography (SFC) is the type of normal phase chromatography that requires superficial fluid as mobile phase like carbon dioxide. It is used for the evaluation and purification of thermally labile molecules low to moderate molecular weight and for the separation of chiral compounds. The principles of super critical fluid chromatography are similar to high performance liquid chromatography, it uses carbon dioxide as the mobile phase, and therefore the pressure should be maintained throughout the entire chromatographic flow path. Sometimes super fluid chromatography is called convergence chromatography because supercritical phase represent a state where gas and liquid properties converge.
- Separation of chiral molecules
- Achiral separations
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