Ionization Technique

There are many Ionization techniques in Mass spectrometry. Electron impact (EI) and Fast Atom Bombardment (FAB) are the classical ionization technique used mainly by the chemist. Now these ionization techniques are not generally used in modern mass spectrometry except EI for environmental work using GC-MS. Atmospheric Pressure Chemical Ionization (APCI), electrospray ionization (ESI), Matrix Assisted Laser Desorption Ionization (MALDI) are Modern Ionization techniques in mass spectrometry. APCI forms a protonated molecule and is completely compatible with Liquid Chromatography (LC) and so it is more preferred over EI methods. ESI Combining with Matrix assisted laser desorption ionization (MALDI) has more or less eliminated the application of FAB, because it produces the protonated molecules, which made FAB very popular, but is much more sensitive. ESI is the ionization technique is one of the most important ionisation technique in which electrospray is created by putting a high voltage on a flow of liquid at atmospheric pressure, sometimes this is done by a concurrent flow of gas. It is easily adapted to liquid chromatography (LC) due to natural use of a flowing liquid. There are many variations in this electrospray technique Such as Nanospray, Static Nanospray, Desorption Electrospray Ionization (DESI) and many more. Similarly, atmospheric pressure photo ionization (APPI) is the variations in APCI where ionization is performed by photo ionization, usually of a dopant that absorbs the light and is added to the sample flow. MALDI is highly utilized in imaging mass spectrometry.

  • Electron Impact ionization (EI)
  • Electrospray ionization technique
  • Atmospheric Pressure Chemical Ionization (APCI)
  • Matrix Assisted Laser Desorption Ionization technique
  • Plasma and glow discharge
  • Field ionization
  • Resonance ionization technique
  • Thermal ionization

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